In situFTIR studies on mammalian cells

Studies on the Division Cycle of Mammalian Cells

In cultures of murine neoplastic mast cells, the duration of different phases of the division cycle (G(1), S, G(2), and mitosis [M]) was determined under optimal and several well-defined suboptimal growth conditions. Two methods of evaluation were applied to the same culture system: first, the relative number of G(1), S, G(2), and M cells was determined by pulse labeling of samples with thymidi...

Overexpression of membrane proteins in mammalian cells for structural studies

The number of structures of integral membrane proteins from higher eukaryotes is steadily increasing due to a number of innovative protein engineering and crystallization strategies devised over the last few years. However, it is sobering to reflect that these structures represent only a tiny proportion of the total number of membrane proteins encoded by a mammalian genome. In addition, the str...

Experimental results on mammalian cells growing in vitro in deuterated medium for neutron-scattering studies.

SV-40 virus-transfromed human diploid fibroblasts (2RA) were grown in a monolayer on plastic Petri dishes in an aqueous medium deuterated to different concentrations of deuterium oxide: 10, 20, 30, up to 60%. The cells must be acclimatized to concentrations higher than 20% D2O by stepping them from a lower initial concentration during their exponential growth. The increase of cell doubling time...

Recent Studies on Experimental Mammalian Leukemia *

Leukemia was first described during the middle of the nineteenth century.4' " Subsequent hematologic and bacteriologic study by numerous investigators demonstrated that human leukemia in none of its forms could be related to any definitive bacterial, animal parasitic, or filterable agent. Much of the descriptive pathological work has centered about the classification of this intriguing disease;...

Expression of Biologically Active Recombinant B-Domain-Deleted Human Factor VIII in Mammalian Cells